Primer

Part:BBa_K360114:Design

Designed by: Mariana Ruiz Velasco Leyva   Group: iGEM10_UNAM-Genomics_Mexico   (2010-10-15)


Primer to change RBS of P. pyralis luciferase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 3
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 3
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 3
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We were not able to carry out an appropriate assay to check whether deleting the insertion was more efficient.


Source

This primer is composed by the prefix, the RBS BBa_B0034, and a spacer of 7 nucleotides.

References